CGSC Strain#: 6365
Strain Designation: RE231     
Source of Strain: R.C. Essenberg
Sex: Hfr
Point of Origin#: 2A
Map Position: 12.20
Direction of Marker Transfer: Counterclockwise
Episome/Plasmid: pLC34-43
Plasmid Markers/Mutations: thr-1,
araC14,
leuB6(Am)
,
lacY1,
glnX44(AS)
,
galK2(Oc)
,
λ-,
mtl-1,
thiE1,
galT22,
recA56,
xylA5,
ΔtrpE63,
uhpB ,
uhpC ,
uhpA ,
ilvB Plasmid Comment: In RE231, #6365. R. Essenberg strain. Hybridizes with Kohara clones 567-569.
Chromosomal Markers: fhuA22,
ompF627(T2R)
,
uhp-40,
recA56,
srlD300::Tn10,
relA1,
pyrE41,
spoT1,
tnaA6,
metB1,
gltSo14Strain Comments: - recA56-- Amino acid substitution Arg 60 Cys
- srlD300::Tn10-- : The insertion was determined by sequencing to be within srlD (F.W.Pons, pers.commun., Oct.2000)
- spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
- gltSo14-- is Halpern's gltC7.
- srlD300::Tn10 was formerly called srlC300::Tn10
- = tetracycline resistant
- T2R = T2 Resistant
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
References: - Essenberg, R.C. 0. Pers. Comm. Pers. Commun. 00:0-0
- Neidhardt, F.C., V. Vaughn, T.A. Phillips, P.L. Bloch 1983. Gene-protein index of Escherichia coli K-12. Microbiol.Rev. 47:231-284