CGSC Strain#: 5015
Strain Designation: Hfr Broda 8      Source of Strain: P. Broda
Sex: Hfr    Point of Origin#: 118   Map Position: 8.21   Direction of Marker Transfer: Clockwise
Chromosomal Markers: λ^-, relA1, spoT1, metB1, creC510, λ^R
Strain Comments:

• spoT1-- In addition to the 6bp insertion, there is a C to T transition resulting in an H257Y substitution.
• creC510-- The constitutive phenotype is due to an R77P amino acid substitution
• λ^R-- Lambda resistant, locus unknown.
• creC510 was formerly called phoM510
• Const = constitutive

References:

• Matney, T.S., E.P. Goldschmidt, N.S. Erwin, R.A. Scroggs 1964. A preliminary map of genomic sites for F-attachment in Escherichia coli K12. Biochem.Biophys.Res.Commun. 17:278-281
• Broda, P. 1967. The formation of Hfr strains in Escherichia coli K12. Genet.Res. 9:35-47