CGSC Strain#: 5823
Strain Designation: JBK248      Source of Strain: J.B. Kirschbaum
Sex: F- Chromosomal Markers: fhuA22, lacY1, glnX44(AS), gal-6, λ^-, hisG1, mgl-50, malT1(λ^R), xyl-7, mtlA2, metB1, rplL9(L?), rpoC110(ts), thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant
• ts = Temperature Sensitive

References:

• Kirschbaum, JB 1978. A mutation in the gene for the beta' subunit of Escherichia coli RNA polymerase which specifically affects transcription of the rpoBC operon. J. Mol. Biol. 119(1):37-47.