CGSC Strain#: 5821
Strain Designation: JBK240      Source of Strain: J.B. Kirschbaum
Sex: F- Chromosomal Markers: fhuA2::IS2, lacY1, glnX44(AS), λ^-, hisG1, malT1(λ^R), xyl-7, mtlA2, metB1, rplL9(L?), thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• L? = assignment uncertain
• LamR = Lambda-resistant

References:

• Kirschbaum, JB, IV Claeys, S Nasi, B Molholt, JH Miller 1975. Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation. Proc. Natl. Acad. Sci. U.S.A. 72(6):2375-9.
• Kirschbaum, JB 1978. A mutation in the gene for the beta' subunit of Escherichia coli RNA polymerase which specifically affects transcription of the rpoBC operon. J. Mol. Biol. 119(1):37-47.