CGSC Strain#: 5822
Strain Designation: JBK246     
Source of Strain: J.B. Kirschbaum
Sex: F-
Chromosomal Markers: fhuA2::IS2,
lacY1,
glnX44(AS)
,
λ-,
hisG1,
rpsL9,
malT1(λ
R)
,
xyl-7,
mtlA2,
metB1,
rpoB2(ts)
,
rpoB7(ts,rifR)
,
thiE1Strain Comments: - glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
- rpoB2(ts)-- is a temperature-sensitive mutation.
- fhuA2::IS2 was formerly called fhuA2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- xyl-7 was formerly called xylA7
- ::IS2 = Insertion of IS2
- AS = amber(UAG) suppressor
- LamR = Lambda-resistant
- rifR = rifamycin resistance
- ts = Temperature Sensitive
References: - Kirschbaum, JB, IV Claeys, S Nasi, B Molholt, JH Miller 1975. Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation. Proc. Natl. Acad. Sci. U.S.A. 72(6):2375-9.