CGSC Strain#: 5822
Strain Designation: JBK246      Source of Strain: J.B. Kirschbaum
Sex: F- Chromosomal Markers: fhuA2::IS2, lacY1, glnX44(AS), λ^-, hisG1, rpsL9, malT1(λ^R), xyl-7, mtlA2, metB1, rpoB2(ts), rpoB7(ts,rifR), thiE1
Strain Comments:

• glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
• hisG1-- This mutation has been reported as either a frameshift or a 36 nt deletion
• rpoB2(ts)-- is a temperature-sensitive mutation.
• fhuA2::IS2 was formerly called fhuA2
• glnX44(AS) was formerly called su⁺_II
• glnX44(AS) was formerly called supE44
• glnX44(AS) was formerly called glnV44
• thiE1 was formerly called thi-1
• xyl-7 was formerly called xylA7
• ::IS2 = Insertion of IS2
• AS = amber(UAG) suppressor
• LamR = Lambda-resistant
• rifR = rifamycin resistance
• ts = Temperature Sensitive

References:

• Kirschbaum, JB, IV Claeys, S Nasi, B Molholt, JH Miller 1975. Temperature-sensitive RNA polymerase mutants with altered subunit synthesis and degradation. Proc. Natl. Acad. Sci. U.S.A. 72(6):2375-9.