CGSC Strain#: 5927
Strain Designation: CM4935     
Source of Strain: W.S. Kelley
Sex: F-
Chromosomal Markers: lacZ53(Am)
,
thyA36,
rpsL151(strR)
,
metE70,
rha-5,
deoC2,
λNM857Strain Comments: - This strain carries λ phage derivative NM857 of N. Murray: λ polAcI857att+ Δnin5Qam73.
- lacZ53(Am)-- is the Paris lac-Y14 amber.
- thyA36-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- Am = amber(UAG) mutation
- strR = streptomycin resist.
References: - Kelley, WS, KH Stump 1979. A rapid procedure for isolation of large quantities of Escherichia coli DNA polymerase I utilizing a lambdapolA transducing phage. J. Biol. Chem. 254(9):3206-10.