CGSC Strain#: 5991
Strain Designation: ST640     
Source of Strain: H. Matsuzawa
Other Designations: H879
Other Designations: ATK121
Other Designations: PC1358
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
ddlB1(ts)
,
secA216,
fhuA61,
lacY1,
galT23,
λ-,
trp-84,
his-215,
rfbC1,
mgl-51,
thyA710,
rpsL263(strR)
,
xylA5,
mtl-1,
thiE1Strain Comments: - We find this strain to be Ts for growth at 44 degrees C, but not at 42 degrees C.
- leuB6(Am)-- Suppressed by serU suppressor (supH)
- galT23-- : This is the standard gal6 of Morse & Lederberg.
- thyA710-- Strains with this marker must have their medium supplemented with thymine (25ug/ml). This is true even for rich medium such as LB. Otherwise thymine starvation can lead to thymineless death.
- rfbC1 was formerly called rfbD1
- secA216 was formerly called azi-16
- secA216 was formerly called secA16
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- strR = streptomycin resist.
- ts = Temperature Sensitive
References: - Matsuzawa, H, M Matsuhashi, A Oka, Y Sugino 1969. Genetic and biochemical studies on cell wall peptidoglycan synthesis in Escherichia coli K-12. Biochem. Biophys. Res. Commun. 36(4):682-9.