CGSC Strain#: 6034
Strain Designation: PAM164     
Source of Strain: B.F. Johnson
Sex: F-
Chromosomal Markers: thr-1,
araC14,
leuB6(Am)
,
Δ(gpt-proA)62,
lacY1,
tsx-33,
lon-22,
glnX44(AS)
,
galK2(Oc)
,
λ-,
sulA28,
hisG4(Oc)
,
rpsL31(strR)
,
xylA5,
mtl-1,
metF24,
thiE1Strain Comments: - leuB6(Am)-- Suppressed by serU suppressor (supH)
- glnX44(AS)-- Mutation is a C to T transition at nucleotide 34 (3rd position of the anticodon)
- galK2(Oc)-- is a GAA(glu) to TAA(ochre) mutation, residue 134. Oller et al.1993. Apparently non-suppressible, since all revertants found were at the ochre site.
- Δ(gpt-proA)62 was formerly called proA2
- galK2(Oc) was formerly called gal2
- glnX44(AS) was formerly called su+II
- glnX44(AS) was formerly called supE44
- glnX44(AS) was formerly called glnV44
- thiE1 was formerly called thi-1
- Am = amber(UAG) mutation
- AS = amber(UAG) suppressor
- Oc = ochre(UAA) mutation
- strR = streptomycin resist.
References: - Donch, J., J. Greenberg 1968. Genetic analysis of lon mutants of strain K-12 of Escherichia coli. Mol.Gen.Genet. 103:105-115
- Johnson, B.F. 1977. Fine structure mapping and properties of mutations suppressing the lon mutation in Escherichia coli K-12 and B strains. Genet.Res. 30:273-286