CGSC Strain#: 6142
Strain Designation: TSM7      Source of Strain: T.J. Silhavy
Sex: F- Chromosomal Markers: [araD139]_B/r, Δ(argF-lac)169, λ^-, flhD5301, Δ(fruK-yeiR)725(fruA25), relA1, rpsL159(strR), rbsR22, malE57::Tn5, Δ(fimB-fimE)632(::IS1), deoC1
Strain Comments:

• [araD139]_B/r-- The thr-leu region was transduced from an E. coli B/r strain (SB3118) in early steps of strain derivation; ara or in some strains ara-leu or thr-leu are from B.
• Δ(argF-lac)169-- extends from mmuP through orfs preceding argF, through lac to mhpD, literally Δ(mmuP-mhpD)169. (Peters et al. 2003 JB 185:2017)
• Δ(argF-lac)169-- from strain Hfr3000 U169 was initially called ΔlacU169 and described as a lacZY mutation until found to include argF and lacI.
• flhD5301-- flagellar defect shown in Komeda and Iino
• Δ(fruK-yeiR)725(fruA25)-- : this fruA25 mutation deletes 29 codons of fruK, fruB and fruBKAp promoter to orf yeiR Peters et al. J.Bact.185:2017.
• Δ(fruK-yeiR)725(fruA25) was formerly called fruA25
• = kanamycin resistant
• ::IS1 = insertion of IS1
• fruA25 = former designation
• strR = streptomycin resist.

References:

• Guarente, L.P., R.R. Isberg, M. Syvanen, T.J. Silhavy 1980. Conferral of transposable properties to a chromosomal gene in Escherichia coli. J.Mol.Biol. 141:235-248