CGSC Strain#: 5096
Strain Designation: CD4      Source of Strain: W. Epstein
Sex: Hfr    Point of Origin#: 2A   Map Position: 12.20   Direction of Marker Transfer: Counterclockwise
Chromosomal Markers: metD-88, Δpro-3, Δ(cod-lacI)6, tsx-76, λ^-, relA1, malT36(λ^R), metB1
Strain Comments:

• Both donor and recipient must be met^-(preferably same mutation) in order to use this marker. Low concentration of D-methionine (5-10ug/ml) works better in selection.
• Δpro-3-- is proA or proB.
• Δ(cod-lacI)6-- is Lederberg's lac^-₇₂ from strain W4032. It is lacI^-Z^-Y^-. Feedback, 1999, indicates that it also deletes part or all of the cod operon.
• Δ(cod-lacI)6 was formerly called Δ6
• Δ(cod-lacI)6 was formerly called lac-3
• Δ(cod-lacI)6 was formerly called lac-72(del)
• metD-88 was formerly called metD1
• LamR = Lambda-resistant

References: