Mutation fadA785(del)::kan
- Name: fadA785(del)::kan
- Type: Insertion
- Mutation of Gene: fadA
- Approx. Map Location: 86.77
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were AGCCAGCCCGTCCGGTTGGCGACCTGAAAACGGCTTAAGGAGTCACAATGATTCCGGGGATCCGTCGACC and AAGGCGTTCACGCCGCATCCGGCAAGTGGTTAAACCCGCTCAAACACCGTTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW5578-1 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, ΔfadA785::kan, Δ(rhaD-rhaB)568, hsdR514