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Strain pLC34-46
- CGSC#: 13665
- Strain Designation
Designation Source Preference pLC34-46 1 - Sex(Hfr,F+,F-,or F'): F+
- 13 Mutations:
Mutation Location Certainty Display thr-1 0.01 1 thr-1 araC14 1.52 1 araC14 leuB6(Am) 1.74 1 leuB6(Am) lacY1 7.79 1 lacY1 glnX44(AS) 14.99 1 glnX44(AS) galK2(Oc) 16.99 1 galK2(Oc) galT22 17.01 1 galT22 LAM- 17.40 1 λ- trpE63(del) 28.44 1 ΔtrpE63 recA56 60.80 1 recA56 xylA5 80.34 1 xylA5 mtl-1 81.26 1 mtl-1 thiE1 90.34 1 thiE1 - Comments:
- Episome/Plasmid: pLC34-46B
Plasmid Markers/Mutations: araC14 galK2(Oc) galT22 glnX44(AS) lacY1 LAM- leuB6(Am) mtl-1 recA56 thiE1 thr-1 trpE63(del) xylA5 nadB trmC ranA ung pssA - References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Raetz, CR, TJ Larson, W Dowhan 1977. Gene cloning for the isolation of enzymes of membrane lipid synthesis: phosphatidylserine synthase overproduction in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 74(4):1412-6.
- Clarke, L., J. Carbon 1979. Selection of specific clones from colony banks by suppression or complementation tests. Methods Enzymol. 68:396-408
- Ohta, T., K. Waggoner, K. Louie, W. Dowhan 1981. Cloning of genes involved in membrane lipid synthesis. Effects of amplification of phosphatidylserine synthetase in Escherichia coli. J.Biol.Chem. 256:2219-2225
- Nishimura, A., K. Akiyama, Y. Kohara, K. Horiuchi 1992. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12. Microbiol.Rev. 56:137-151
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