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Strain E111

• CGSC#: 5393
• Strain Designation

  Designation	Source	Preference
  E111	Wu H.C.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 12 Mutations:

  Mutation	Location	Certainty	Display
  DE(gpt-proA)62	5.51	1	Δ(gpt-proA)62
  lacY1	7.79	1	lacY1
  tsx-33	9.28	1	tsx-33
  galE28(GalS)	17.03	1	galE28(GalS)
  LAM-	17.40	1	λ-
  rpsL31(strR)	74.84	1	rpsL31(strR)
  kdgK51	79.26	1	kdgK51
  xylA5	80.34	1	xylA5
  mtl-1	81.26	1	mtl-1
  glmS1	84.27	1	glmS1
  argE3(Oc)	89.48	1	argE3(Oc)
  thiE1	90.34	1	thiE1

  
  
• Comments:
  • There have been reports that the glmS marker in this strain is leaky or has reverted
  • This strain must be grown on 200ug/ml N-acetylglucosamine, preferably in PPBE medium (see paper cited below).
• References:
  • Wu, HC, TC Wu 1971. Isolation and characterization of a glucosamine-requiring mutant of Escherichia coli K-12 defective in glucosamine-6-phosphate synthetase. J. Bacteriol. 105(2):455-66.
  
  

  Printable Strain Report