Mutation - rsgA787(del)::kan

Mutation - rsgA787(del)::kanMutation rsgA787(del)::kan

Mutation rsgA787(del)::kan

Name: rsgA787(del)::kan
Type: Insertion
Mutation of Gene: rsgA
Approx. Map Location: 94.59
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were GACGATTCTAACGACGGTTAGCTTAATTGTCAGTCATCCGTATCAGAAAAATTCCGGGGATCCGTCGACC and GTATCATAGATGTTTTGCCCATCAGGGGCGACCAGGAGTCTGTACGATTGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11

Property	Symbol
deletion	del
kanamycin resistant

2 Strains Carrying This Mutation

Name	Mutations	Genotype
JW4122-3	7	F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, rph-1, Δ(rhaD-rhaB)568, ΔrsgA787::kan, hsdR514
WTZ19001	9	F-, ara-600, Δ(gpt-lac)5, λ^-, zdi-925::Tn10, infC362, relA1?, spoT1?, thiE1, ΔrsgA787::kan