E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

About the CGSC

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Searching the CGSC Database

• Help With Query Forms
• Strain Query
• Mutation Query
• Gene/Site Query
• Gene Product Query
• Reference Query
• Query Help
• Info on Genetic Nomenclature

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Other CGSC Information

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• Current Working Map
• 1998 MMBR Map
• 1998 MMBR Gene List
• Map Diagrams (PDFs)

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Strain Query

• CGSC#:
• Strain Designation
  
• Source Person (e.g. "Lederberg")
• Sex: Hfr F+ F- F'
• Hfr Point of Origin #:
• Hfr Location (in a clockwise range of minutes) From: To:
• Hfr Direction of Marker Transfer: Clockwise Counterclockwise
• With Mutations and and and
  
• But Not With Mutations or or or
  
• Episome/Plasmid:
• Episome/Plasmid Markers:
• Special Use Strains & Sets: amber suppression mutagenesis araB strain araBAD strain araBp(pBAD) homog.express.set araC strain araC(Const) strain araD strain argA strain argB mutant argC strain argI del.strain aroA strain B wildtype, std. bioA strain bioB strain classic LAM indicator cloning cloning tandem deletions cloning unstable DNA in ColE1 Col plasmid host complementation atpF469 (Rec-) complementation atpG (Rec-) complementation atpH (Rec-) CRIM Host Strains CRIM helper plasmid strains CRIM plasmid strains CRIM lacZ transcr.fusion crp plasmid cya strain cyaA plasmid cysE strain cysH strain dam dcm dam dcm F' for M13 cloning dam dcm hsdR dam dcm recF hsdR dam hsdS dam recF dam- dcm- hsdS- strain dam- endA- rna- dapE mutant dcm hsdR dcm strain deep rough mutant dnaA strain dnaB strain dnaC strain eno- strain F-Prime kit ftsZ strain galE strain galK strain gene disruption set gene disruption set industry Like pKD46, but CmR Like pKD46, except TetR gfp gene integrated glnA strain Glt auxotroph gltA strain gltB strain (needs gdh) glycerol-requiring strain gyrA strain Hfr kit Hfr Tn10 kit Hfr Tn10, Gross hisA hisB strain hisC strain hisD strain hisF strain hisG strain hisH,LamR strain hisI strain host for pir-dependent plasmid host,non-pir plasmid replic. ilvA strain insertion mutagenesis labelling with C13 glycerol lac fusion-stat.phase promoter lac fusions LAM- wildtype LAM-defective leuA strain leuB strain leuC strain leuD strain lon transfer low Thy requirer LPS inner core def.-deeprough lysA strain maxi-cell producer met- strain metA strain metG strain mini-cell producer nagB strain overproduces RNaseI, periplasm P1 lysogen pabA strain pabB strain panD strain pheA strain pheA::Tn10 strain phoA strain phr deletion protease- strain ptsG strain ptsH strain ptsI strain purA strain purA::MudAmp stable purB strain purC strain purE strain pyrB strain pyrC strain pyrD strain pyrE strain pyrF287 strain recBC sbcBC RNase I negative rpoA strain rpoB strain rpoC strain rpoD strain rpoH strain serA F- strain serA Hfr strain serB strain sod double mutant sodA strain sodB strain substitute for DH5alpha supL(lysT) F- option supL2(lysT102) strain teaching F- teaching F-, ATCC kit teaching Hfr teaching lac thyA deo: Labelling in Thy- thymidine labelling transaminase-minus,(labeling) transfer hsdR transfer lac deletion transfer recA+ transfer recA- transformation trpA strain trpA(Am,Oc,or UGA) on F' trpB strain trpC strain trpE deletion trpE strain tyrA non Tn10 mutation tyrA::Tn10 uidA strain very UV sensitive wildtype K-12
• Has Partner Isogenic With Respect to Marker:
• Isogenic Partner Is:
• Comments:
• References: (e.g. "%Wanner%2000")