Mutation - thiD728(del)::kan

Mutation - thiD728(del)::kanMutation thiD728(del)::kan

Mutation thiD728(del)::kan

Name: thiD728(del)::kan
Type: Insertion
Mutation of Gene: thiD
Approx. Map Location: 47.03
Properties
Property Symbol
deletion del
kanamycin resistant
Comments
- The primers used to make this knockout were CAATAACCAAAGCAGCTATAGTACGGTGCTCACCACCAGGCGTGGAAGTGATTCCGGGGATCCGTCGACC and CCACATTTCCTTGATGCGCTCTGGCAATTGACGCAGGAGGTGCAGGCATGTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
References
- Baba, T., T. Ara, M. Hasegawa, Y. Takai, Y. Okumura, M. Baba, K.A. Datsenko, M. Tomita, B.L. Wanner, H. Mori 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:1-11
1 Strains Carrying This Mutation
Name Mutations Genotype
JW2090-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ^-, ΔthiD728::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514

Property	Symbol
deletion	del
kanamycin resistant