Mutation iaaA728(del)::kan
- Name: iaaA728(del)::kan
- Type: Insertion
- Mutation of Gene: iaaA
- Approx. Map Location: 18.66
- Properties
Property Symbol deletion del kanamycin resistant - Comments
- The primers used to make this knockout were TGATATTTATAGCAAAAGTGGCGAACCACCCTTAATGGACGAATACTATGATTCCGGGGATCCGTCGACC and CCGCCAGCACATTACCGGCATCAAGTTCATCACTGTGTGGCAACGGTGTCTGTAGGCTGGAGCTGCTTCG the bolded sequence matches the pKD13 template
- References
- 1 Strains Carrying This Mutation
Name Mutations Genotype JW0812-2 7 F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, ΔiaaA728::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514