Mutation - LAMpir

Mutation - LAMpirMutation LAMpir

Mutation LAMpir

Name: LAMpir
Type: Insertion
Approx. Map Location: 17.40
Comments
- Allows the replication of plasmids with an R6Kγ origin of replication
References
- McEachern, M.J., M. Filutowicz, D.R. Helinski 1985. Mutations in direct repeat sequences and in a conserved sequence adjacent to the repeats result in a defective replication origin in plasmid R6K. Proc.Natl.Acad.Sci.USA 82:1480-1484

4 Strains Carrying This Mutation

Name	Mutations	Genotype
S17-1 λpir/pLFT	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pLFT
S17-1 λpir/pLFX	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pLFX
S17-1 λpir/pUV5	8	F-, RP4-2(Km::Tn7,Tc::Mu-1), pro-82, LAMpir, recA1, endA1, thiE1, hsdR17, creC510, pUV5
VPE42	13	F-, thr-1, leuB6(Am), zad-2084::Tn10, pcnB80, fhuA21?, cyn-101, lacY1, glnX44(AS), LAMpir, rfbC1, recA2111::RP4-2Tc::Mu, glpR200(glp^c), thiE1