Site rfaD
- Name: rfaD
- Type: Gene
- Synonyms
b3619 MG1655 locus tag htrM - Mnemonic: Rough
- Left End Point: 81.73
- Right End Point: 81.75
- Products:
D-glycero-D-mannoheptose epimerase ADP-L-glycero-D-mannoheptose-6-epimerase - Direction: >
- Properties:
Property Comment heat-inducible NULL - Priority: 1
- 3 Alleles of This Gene
rfaD70 rfaD731(del)::kan rfaD791-YFP(::cat) - External Database Links:
Host Site Page Links EcoGene.org EG10838 EcoliWiki rfaD - Comment:
- LPS biosynthesis.(ADP-L-glycerol-D-mannoheptose is a lipopolysac.precursor).Mutation results in altered lipo- polysacc.of D-glycero.,not L-. Defect.LPS = OMPs not protected from degrad.during folding
- References:
- Makela, P.H., B. Stocker 1969. Genetics of polysaccharide biosynthesis. Annu.Rev.Genet. 3:291-322
- Coleman, W.G., L. Leive 1979. Two mutations which affect the barrier function of the Escherichia coli K-12 outer membrane. J.Bacteriol. 139:899-910
- Coleman, W.G. 1983. The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis. J.Biol.Chem. 258:1985-1990
- Coleman, WG, KS Deshpande 1985. New cysE-pyrE-linked rfa mutation in Escherichia coli K-12 that results in a heptoseless lipopolysaccharide. J. Bacteriol. 161(3):1209-14.
- Pegues, J.C., L.S. Chen, A.W. Gordon, L. Ding, W.G. Coleman 1990. Cloning, expression, and characterization of the Escherichia coli K-12 rfaD gene. J.Bacteriol. 172:4652-4660
- Schnaitman, C.A., C. Parker, J.D. Klena, E. Pradel, N.B. Pearson, K.E. Sanderson, P.R. MacLachlan 1991. Physical maps of the rfa loci of Escherichia coli K-12 and Salmonella typhimurium. J.Bacteriol. 173:7410-7411
- Raina, S., C. Georgopoulos 1991. The htrM gene, whose product is essential for Escherichia coli viability only at elevated temperatures, is identical to the rfaD gene. Nucleic Acids Res. 19:3811-3819
- Roncero, C., M.J. Casadaban 1992. Genetic analysis of the genes involved in synthesis of the lipopolysaccharide core in Escherichia coli K-12. J.Bacteriol. 174:3250-3260
- Klena, J.D., E. Pradel, C.A. Schnaitman 1992. Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium. J.Bacteriol. 174:4746-4752
- Schnaitman, CA, JD Klena 1993. Genetics of lipopolysaccharide biosynthesis in enteric bacteria. Microbiol. Rev. 57(3):655-82.
- Sofia, H.J., V.D. Burland, D.L. Daniels, G. Plunkett, F.R. Blattner 1994. Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucleic Acids Res. 22:2576-2586
- Missiakas, D., J.M. Betton, S. Raina 1996. New components of protein folding in extracytoplasmic compartments of Escherichia coli SurA, FkpA and Skp/OmpH. Mol.Microbiol. 21:871-884
- Morrison, J.P., J.A. Read, W.G. Coleman, M.E. Tanner 2005. Dismutase activity of ADP-L-glycero-D-manno-heptose 6-epimerase: evidence for a direct oxidation/reduction mechanism. Biochemistry 44:5907-5915