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Site putP
- Name: putP
- Type: Gene
- Synonyms
b1015 MG1655 locus tag - Mnemonic: proline utilization
- Left End Point: 23.25
- Right End Point: 23.28
- Products:
major proline permease proline/Na+,Li+ symport protein - Direction: >
- Properties:
Property Comment encodes membrane protein NULL transport gene NULL - Priority: 1
- 3 Alleles of This Gene
putP5::Tn5 putP760(del)::kan putP792-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank X05653 EcoGene.org EG10802 EcoliWiki putP - Comment:
- Sel. phen. Loss: putA-, P-, azetidine carboxylate res. (still dehydroproline sens.); putA+, P-, azetidine carboxy., dehydro- proline resistance; indicator plate putP-, dehydroproline res.
- References:
- Tristram, H., S. Neale 1968. The activity and specificity of the proline permease in wild-type and analogue-resistant strains of Escherichia coli. J.Gen.Microbiol. 50:121-137
- Wood, J.M., D. Zadworny 1979. Characterization of an inducible porter required for L-proline catabolism by Escherichia coli K12. Can.J.Biochem. 57:1191-1199
- Wood, J.M., D. Zadworny 1980. Amplification of the put genes and identification of the put gene products in Escherichia coli K12. Can.J.Biochem. 58:787-796
- Wood, J.M. 1981. Genetics of L-proline utilization in Escherichia coli. J.Bacteriol. 146:895-901
- Stalmach, M.E., S.R. Grothe, J.M. Wood 1983. Two proline porters in Escherichia coli K-12. J.Bacteriol. 156:481-486
- Rancourt, D.E., J.T. Stephenson, G.A. Vickell, J.M. Wood 1984. Proline excretion by Escherichia coli K12. Biotechnol.Bioeng. 26:74-80
- Mogi, T., H. Yamamoto, T. Nakao, I. Yamato, Y. Anraku 1986. Genetic and physical characterization of putP, the proline carrier gene of Escherichia coli K12. Mol.Gen.Genet. 202:35-41
- Nakao, T., I. Yamato, Y. Anraku 1987. Nucleotide sequence of putC, the regulatory region for the put regulon of Escherichia coli K12. Mol.Gen.Genet. 210:364-368
- Nakao, T., I. Yamato, Y. Anraku 1987. Nucleotide sequence of putP, the proline carrier gene of Escherichia coli K12. Mol.Gen.Genet. 208:70-75
- Ohsawa, M., T. Mogi, H. Yamamoto, I. Yamato, Y. Anraku 1988. Proline carrier mutant of Escherichia coli K-12 with altered cation sensitivity of substrate-binding activity: cloning, biochemical characterization and identification of the mutation. J.Bact
- Yamato, I., M. Ohsawa, Y. Anraku 1990. Defective cation-coupling mutants of Escherichia coli Na+/Proline symport carrier. J.Biol.Chem. 265:2450-2455