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Site nusA
- Name: nusA
- Type: Gene
- Synonyms
b3169 MG1655 locus tag - Mnemonic: Nu (lambda protein) survival
- Left End Point: 71.43
- Right End Point: 71.46
- Products:
transcription termination L factor - Direction: <
- Properties:
Property Comment transcription term./antiterm. nusA effects both termination and antitermination; couples factors to RNAP for elongations. control. controls pausing, allowing envir. factors to interact. - Priority: 1
- 2 Alleles of This Gene
nusA1 nusA791-YFP(::cat) - External Database Links:
Host Site Page Links NCBI/GenBank M26839 NCBI/GenBank X00513 EcoGene.org EG10665 EcoliWiki nusA - Comment:
- Selectable phenotype. Survival of induction of mutant LAM prophage. NusA binds to both RNAP and Lambda protein N, forming antiterminator complex.also binds Rho,stimul.term.Also stimul.antiterm. by Q.
- References:
- Friedman, D.I. 1971. A bacterial mutant affecting lambda development, p.733-738. In A.D. Hershey (ed.), The bacteriophage lambda. Cold Spr.Harb.Symp.Quant.Biol. 1:733-738
- Greenblatt, J., J. Li, S. Adhya, D.I. Friedman, L.S. Baron, B. Redfield, H.-F. Kung, H. Weissbach 1980. L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination. Proc.Natl.Acad.Sci.USA 77
- Ward, D.F., M.E. Gottesman 1981. The nus mutations affect transcription termination in Escherichia coli. Nature 292:212-215
- Greenblatt, J., M. McLimont, S. Hanly 1981. Termination of transcription by nusA gene protein of Escherichia coli. Nature 292:215-220
- Plumbridge, J.A., J.G. Howe, M. Springer, D. Touati-Schwartz, J.W.B. Hershey, M. Grunberg-Manago 1982. Cloning and mapping of a gene for translation initiation factor 1F2 in Escherichia coli. Proc.Natl.Acad.Sci.USA 79:5033-5037
- Plumbridge, J.A., M. Springer 1983. Organization of the Escherichia coli chromosome around the genes for translation initiation factor 1F2 (infB) and a transcription termination factor (nusA). J.Mol.Biol. 167:227-243
- Kurihara, T., H. Nakamura 1983. Cloning of the nusA gene of Escherichia coli. Mol.Gen.Genet. 190:189-195
- Ishii, S., K. Kuroki, F. Imamoto 1984. tRNA$_{f2}$$^{Met}$ gene in the leader region of the nusA operon in Escherichia coli. Proc.Natl.Acad.Sci.USA 81:409-413
- Ishii, S., M. Ihara, T. Maekawa, H. Nakamura, H. Uchida, F. Imamoto 1984. The nucleotide sequence of the cloned nusA gene and its flanking region of Escherichia coli. Nucleic Acids Res. 12:3333-3342
- Nakamura, H., S. Mizusawa 1985. In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins. EMBO J. 4:527-532
- Greer, S., R.N. Perham 1986. Glutathione reductase from Escherichia coli: cloning and sequence analysis of the gene and relationship to other flavoprotein disulfide oxidoreductases. Biochemistry 25:2736-2742
- Saito, M., A. Tsugawa, K. Egawa, Y. Nakamura 1986. Revised sequence of the nusA gene of Escherichia coli and identification of nusA11 (ts) and nusA1 mutations which cause changes in a hydrophobic amino acid cluster. Mol.Gen.Genet. 205:380-382
- Tsugawa, A., M. Saito, D.L. Court, Y. Nakamura 1988. nusA amber mutation that causes temperature-sensitive growth of Escherichia coli. J.Bacteriol. 170:908-915
- Linn, T., J. Greenblatt 1992. The NusA and NusG proteins of Escherichia coli increase the in vitro readthrough frequency of a transcriptional attenuator preceding the gene for the beta subunit of RNA polymerase. J.Biol.Chem. 267:1449-1454
- Das, A. 1992. How the phage Lambda N gene product suppresses transciption termination: communication of RNA polymerase with regulatory proteins mediated by signals in nascent RNA. J.Bacteriol. 174:6711-6716
- Zhang, Y., M.M. Hanna 1994. NusA changes the conformation of Escherichia coli RNA polymerase at the binding site for the 3' end of the nascent RNA. J.Bacteriol. 176:1787-1789