Site trpA
- Name: trpA
- Type: Gene
- Synonyms
b1260 MG1655 locus tag - Mnemonic: tryptophan
- Left End Point: 28.33
- Right End Point: 28.35
- Products:
tryptophan synthase subunit A - Direction: <
- Properties:
Property Comment auxotrophy-amino acid NULL - Priority: 1
- External Database Links:
Host Site Page Links EcoGene.org EG11024 EcoliWiki trpA - Comment:
- 90 map 28.0; rudd coord.1324.6, 1325.4; 28.18, 28.2. Sel. phenotype. Operon: deleted with tonB; deleted with opp. Const.: 5-methyltrptophan; 6-fluorotryptophan res.
- References:
- Guest, J.R., G.R. Drapeau, B.C. Carlton, C. Yanofsky 1967. The amino acid sequence of the A protein (alpha subunit) of the tryptophan synthetase of Escherichia coli. J.Biol.Chem. 242:5442-5446
- Hiraga, S. 1969. Operator mutants of the tryptophan operon in Escherichia coli. J.Mol.Biol. 39:159-179
- Gottesman, S., J.R. Beckwith 1969. Directed transposition of the arabinose operon: a technique for the isolation of special transducing bacteriophages for any Escherichia coli gene. J.Mol.Biol. 44:117-127
- Mattern, I.E., J. Pittard 1971. Regulation of tyrosine biosynthesis in Escherichia coli K-12: isolation and characterization of operator mutants. J.Bacteriol. 107:8-15
- Nichols, B., C. Yanofsky 1979. Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison. Proc.Natl.Acad.Sci.USA 76:5244-5248
- Enger-Valk, B., H. Heyneker, R.A. Oosterbaan, P.H. Pouwels 1980. Construction of new cloning vehicles with genes of the tryptophan operon of Escherichia coli as genetic markers. Gene 9:69-85
- Wu, A.M., A.B. Chapman, T. Platt, L.P. Guarente, J.R. Beckwith 1980. Deletions of distal sequence affect termination of transcription at the end of the tryptophan operon in E. coli. Cell 19:829-836
- Wu, A.M., G.E. Christie, T. Platt 1981. Tandem termination sites in the tryptophan operon of Escherichia coli. Proc.Natl.Acad.Sci.USA 78:2913-2917
- Yanofsky, C., T. Platt, I.P. Crawford, B. Nichols, G.E. Christie, H. Horowitz, M. VanCleemput, A.M. Wu 1981. The complete nucleotide sequence of the tryptophan operon of E. coli. Nucleic Acids Res. 9:6647-6668
- Swift, S., J. Kuhn, G.S. Stewart 1992. Selection and analysis of non-interactive mutants in the Escherichia coli tryptophan synthase alpha subunit. Mol.Gen.Genet. 233:129-135
- Pogliano, J.A., J.R. Beckwith 1994. SecD and SecF facilitate protein export in Escherichia coli. EMBO J. 13:554-561