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Site pLD55
- Name: pLD55
- Type: Plasmid
- Plasmid Sites:
bla-1(ApR,dPstI) MCS(SK) ori f1,+ oriR6Kgamma oriT RP4 tetA tetR - Priority: 0
- Comment:
- {\it lacZ$\alpha$ - ori$_{f1,+}$} region is from pBluescript II(SK+). See Alting-Mees reference below.
- References:
- Alting-Mees, M.A., J.A. Sorge, J.M. Short 1992. pBluescriptII: Multifunctional cloning and mapping vectors. Methods Enzymol. 216:483-495
- Metcalf, WW, W Jiang, LL Daniels, SK Kim, A Haldimann, BL Wanner 1996. Conditionally replicative and conjugative plasmids carrying lacZ alpha for cloning, mutagenesis, and allele replacement in bacteria. Plasmid 35(1):1-13.
- 1 Strains Carrying This Plasmid
Name Mutations Genotype BW21038/pLD55 6 F-, Δ(codB-lacI)3, ΔphoA532, ΔuidA3::pir+, recA1, Δ(phnP-phnD)3330( phnC?), mini-Tn5Lac4(CamR,lacIQ), pLD55