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Strain - BB26-36Strain BB26-36Strain BB26-36
- CGSC#: 5348
- Strain Designation
- Sex(Hfr,F+,F-,or F'): Hfr
- Hfr Point of Origin#: 2A
- PO Map Position: 12.20
- Direction of Marker Transfer: Counterclockwise
- 15 Mutations:
- Comments:
- This strain is not longer has the G-3-P auxotrophy of its parent strain BB26, because of the loss of fb inhibition of GlpK by Fru-1,6-diP, so it can produce G-3-P from glycerol and grow on minimal plus glycerol plus glucose.
- References:
- Bell, RM 1974. Mutants of Escherichia coli defective in membrane phospholipid synthesis: macromolecular synthesis in an sn-glycerol 3-phosphate acyltransferase Km mutant. J. Bacteriol. 117(3):1065-76.
- McIntyre, TM, BK Chamberlain, RE Webster, RM Bell 1977. Mutants of Escherichia coli defective in membrane phospholipid synthesis. Effects of cessation and reinitiation of phospholipid synthesis on macromolecular synthesis and phospholipid turnover. J. Biol. Chem. 252(13):4487-93.
- Larson, T.J., D.N. Ludtke, R.M. Bell, S. Jackowski 1984. sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia coli: evidence that a second mutation, plsX, is required. J.Bacteriol. 160:711-717