- CGSC#: 12351
- Strain Designation
Designation Source Preference DE418 Stahl F. 1 DE418/pLM2 Stahl F. 2
- Sex(Hfr,F+,F-,or F'): F-
- 6 Mutations:
Mutation Location Certainty Display LAM- 17.40 0 λ- thyA36 63.86 0 thyA36 IN(rrnD-rrnE)1 73.74 0 IN(rrnD-rrnE)1 rph-1 82.20 0 rph-1 rpoB331(rifR) 90.08 0 rpoB331(rifR) hsdR19 98.74 0 hsdR19
- It is believed that this plasmid is in a W3110 background, but that has not been positively established. It is documented to be a non-suppressing host so it should be sensitive to ampicillin and tetracycline.
- Episome/Plasmid: pLM2
Plasmid Markers/Mutations: ampR(Am) kan tet(Am) kan
- Mindich, L., C.J. Cohen, M. Weisburd 1976. Isolation of nonsense suppressor mutants of Pseudomonas. J.Bacteriol. 126:177-182
- Seed, B 1983. Purification of genomic sequences from bacteriophage libraries by recombination and selection in vivo. Nucleic Acids Res. 11(8):2427-45.