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Strain BW25141/pKD4

• CGSC#: 7632
• Strain Designation

  Designation	Source	Preference
  BW25141/pKD4	Wanner B.L.	1
  pKD4		2

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 11 Mutations:

  Mutation	Location	Certainty	Display
  DE(araD-araB)567	1.40	1	Δ(araD-araB)567
  lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
  DE(phoB-phoR)580	8.96	1	Δ(phoB-phoR)580
  LAM-	17.40	1	λ-
  galU95	27.82	1	galU95
  uidA3(del)::pir+	36.48	1	ΔuidA3::pir+
  recA1	60.80	1	recA1
  endA9(del-ins)::FRT	66.57	1	endA9(del-ins)::FRT
  rph-1	82.20	1	rph-1
  DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
  hsdR514	98.74	1	hsdR514

  
  
• Comments:
  • This strain should be grown on rich media with ampicillin (100 ug/ml), kanamycin (50 ug/ml) or both at 37 C. pKD4 sequence: GenBank AY048743.
• Episome/Plasmid: pKD4

  Plasmid Markers/Mutations:
  bla(ApR)
  kan
  oriR6Kgamma
  rgnB(Ter)
  bla
  FRT
  kan

• References:
  • Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
  
  

  Printable Strain Report