E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain A19

• CGSC#: 5997
• Strain Designation

  Designation	Source	Preference
  A19	Gesteland R.F.	1

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 21
• PO Map Position: 97.00
• Direction of Marker Transfer: Clockwise
• 6 Mutations:

  Mutation	Location	Certainty	Display
  rna-19	13.87	1	rna-19
  gdhA2	39.67	1	gdhA2
  his-95	45.01	0	his-95
  relA1	62.71	1	relA1
  spoT1	82.34	1	spoT1
  metB1	88.94	1	metB1

  
  
• Comments:
  • The his marker may have reverted in CGSC #5997.
  • The strain has been reported by Reiner to have an unidentified growth requirement satisfied by small amounts of yeast extract.
• References:
  • Clark, A.J. 1963. Genetic analysis of a "double male" strain of Escherichia coli K-12. Genetics 48:105-120
  • Gesteland, RF 1966. Isolation and characterization of ribonuclease I mutants of Escherichia coli. J. Mol. Biol. 16(1):67-84.
  • Reiner, AM 1969. Genetic locus for ribonuclease I in Escherichia coli. J. Bacteriol. 97(3):1522-3.
  
  

  Printable Strain Report