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Strain DF40

• CGSC#: 4871
• Strain Designation

  Designation	Source	Preference
  DF40	Fraenkel D.	1
  L40		3

  
  
• Sex(Hfr,F+,F-,or F'): Hfr
• Hfr Point of Origin#: 2A
• PO Map Position: 12.20
• Direction of Marker Transfer: Counterclockwise
• 11 Mutations:

  Mutation	Location	Certainty	Display
  garB10	3.60	1	garB10
  fhuA22	3.61	1	fhuA22
  ompF627(T2R)	21.24	1	ompF627(T2R)
  fadL701(T2R)	53.01	1	fadL701(T2R)
  relA1	62.71	1	relA1
  pitA10	78.36	1	pitA10
  spoT1	82.34	1	spoT1
  rrnB-2	89.76	1	rrnB-2
  pgi-2	91.21	1	pgi-2
  mcrB1	98.63	1	mcrB1
  creC510	99.89	1	creC510

  
  
• Comments:
  • We maintain this strain on a minimal medium containing 25 mM malate, 12.5 mM glycerol, and 0.1% casamino acids (Hillman and Fraenkel, 1975, J.Bacteriol.122:1175)
• References:
  • Fraenkel, DG, SR Levisohn 1967. Glucose and gluconate metabolism in an Escherichia coli mutant lacking phosphoglucose isomerase. J. Bacteriol. 93(5):1571-8.
  • Fraenkel, DG 1967. Genetic mapping of mutations affecting phosphoglucose isomerase and fructose diphosphatase in Escherichia coli. J. Bacteriol. 93(5):1582-7.
  
  

  Printable Strain Report