E. coli Genetic Resources at Yale
CGSC, The Coli Genetic Stock Center

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Strain EB106

• CGSC#: 6024
• Strain Designation

  Designation	Source	Preference
  EB106	Birge E.A.	1

  
  
• Sex(Hfr,F+,F-,or F'): F-
• 6 Mutations:

  Mutation	Location	Certainty	Display
  LAM-	17.40	1	λ-
  icd-11	25.75	1	icd-11
  dadA1(op)	26.66	1	dadA1(op)
  trpA62	28.33	1	trpA62
  trpE61	28.44	1	trpE61
  tnaA5	83.77	1	tnaA5

  
  
• Comments:
  • This strain should be maintained on minimal medium with Tryptophan and 350 ug/ml glutamate, in order to avoid selecting for gltA mutations. The icd mutation confers a Nal^R phenotype.
• References:
  • Apostolakos, D., P.A. Menter, B.J. Rampsch, H.C. Reeves, E.A. Birge 1982. Genetic map position of the cistron coding for isocitrate dehydrogenase in Escherichia coli K-12. Curr.Microbiol. 7:45-47
  
  

  Printable Strain Report