Mutation - LAMDE3

Mutation - LAMDE3Mutation LAMDE3

Mutation LAMDE3

Name: LAMDE3
Type: Insertion
Approx. Map Location: 17.38
Comments
- Lambda DE3 has the phage T7 RNA polymerase under the control of the lacUV5 promoter inserted into the BamHI site of the Lambda cloning vector D69.
References

7 Strains Carrying This Mutation

Name	Mutations	Genotype
BA-002	3	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, ?ptsI845::FRT, [mal⁺]_K-12(λ^S), hsdS10
HMS174(DE3)	6	F-, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], recA1, IN(rrnD-rrnE)1, rph-1?, rpoB331(rifR), hsdR19
HMS174(DE3)/pLysS	6	F-, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], recA1, IN(rrnD-rrnE)1, rph-1?, rpoB331(rifR), hsdR19, pLysS
BL21(DE3)	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal⁺]_K-12(λ^S), hsdS10
BL21(DE3)/pCP20	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal⁺]_K-12(λ^S), hsdS10, pCP20
BL21(DE3)/pKD46	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal⁺]_K-12(λ^S), hsdS10, pKD46
BL21(DE3)/pLysS	7	F-, lon-11, Δ(ompT-nfrA)885, Δ(galM-ybhJ)884, λDE3 [lacI, lacUV5-T7 gene 1, ind1, sam7, nin5], Δ46, [mal⁺]_K-12(λ^S), hsdS10, pLysS