Strain - VS105

Strain - VS105Strain VS105

Strain VS105

CGSC#: 8178
Strain Designation
Designation Source Preference
VS105 Stupina V.A. 1
Sex(Hfr,F+,F-,or F'): F-
5 Mutations:
Mutation Location Certainty Display
LAM- 17.40 1 λ^-
lacZp-topA76(L5F C662H) 28.70 1 lacZp-topA76(L5F C662H)
zci-2234::cat 28.71 1 zci-2234::cat
fnr-267(del) 30.11 0 Δfnr-267
rph-1 82.20 1 rph-1
Comments:
- This strain will grow at 37-42 C in LB + 15 ug/ml chloramphenicol, but for growth at 30C, it also requires 1 mM IPTG
References:
- Stupina, V.A., J.C. Wang 2005. Viability of Escherichia coli topA mutants lacking DNA topoisomerase I. J.Biol.Chem. 280:355-360
Printable Strain Report

Mutation	Location	Certainty	Display
LAM-	17.40	1	λ^-
lacZp-topA76(L5F C662H)	28.70	1	lacZp-topA76(L5F C662H)
zci-2234::cat	28.71	1	zci-2234::cat
fnr-267(del)	30.11	0	Δfnr-267
rph-1	82.20	1	rph-1