Mutation - lacZp-topA76(L5F C662H)

Mutation - lacZp-topA76(L5F C662H)Mutation lacZp-topA76(L5F C662H)

Mutation lacZp-topA76(L5F C662H)

Name: lacZp-topA76(L5F C662H)
Type: Splice
Approx. Map Location: 28.70
Properties
Property Symbol
amino acid changes in TopoI L5F C662H
Comments
- : poor growth at 30C in absence of IPTG; At 42C, TopoI level is low and more negative supercoiling is observed, but the strain grows if it is topB⁺.
- replaces promoter topAp of allele topA78(G65N W79S substitutions in TopoI) with lacZp, resulting in an IPTG requirement for expression.
References

Property	Symbol
amino acid changes in TopoI	L5F C662H

4 Strains Carrying This Mutation

Name	Mutations	Genotype
VS105	5	F-, λ^-, lacZp-topA76(L5F C662H), zci-2234::cat, Δfnr-267?, rph-1
VS106	6	F-, λ^-, lacZp-topA76(L5F C662H), zci-2234::cat, Δfnr-267?, topB77::Tn5, rph-1
VS101	17	F-, Δ(gpt-proA)62, lacY1, tsx-29, glnX44(AS)?, galK2(Oc), λ^-, lacZp-topA76(L5F C662H), zci-2234::cat, cysB38, Rac-0, hisG4(Oc), rfbC1, kdgK51, xylA5, mtl-1, argE3(Oc), thiE1
VS102	18	F-, Δ(gpt-proA)62, lacY1, tsx-29, glnX44(AS)?, galK2(Oc), λ^-, lacZp-topA76(L5F C662H), zci-2234::cat, cysB38, Rac-0, topB77::Tn5, hisG4(Oc), rfbC1, kdgK51, xylA5, mtl-1, argE3(Oc), thiE1