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Strain pLC25-33/WL8
- CGSC#: 6595
- Strain Designation
Designation Source Preference pLC25-33/WL8 Walsh J. 1 - Sex(Hfr,F+,F-,or F'): F-
- 6 Mutations:
Mutation Location Certainty Display recA56 60.80 1 recA56 srlD300::Tn10 60.91 1 srlD300::Tn10 relA1 62.71 1 relA1 spoT1 82.34 1 spoT1 metB1 88.94 1 metB1 melA7 93.54 1 melA7 - Comments:
- Genes identified on plasmid pLC25-33: melA, melB, fumB
- Episome/Plasmid: pLC25-33
Plasmid Markers/Mutations: araC14 galK2(Oc) galT22 glnX44(AS) lacY1 LAM- leuB6(Am) mtl-1 recA56 thiE1 thr-1 trpE63(del) xylA5 melA melB fumB - References:
- Clarke, L, J Carbon 1976. A colony bank containing synthetic Col El hybrid plasmids representative of the entire E. coli genome. Cell 9(1):91-9.
- Tsuchiya, T, K Ottina, Y Moriyama, MJ Newman, TH Wilson 1982. Solubilization and reconstitution of the melibiose carrier from a plasmid-carrying strain of Escherichia coli. J. Biol. Chem. 257(9):5125-8.
- Guest, J.R., J.S. Miles, R.E. Roberts, S.A. Woods 1985. The fumarase genes of Escherichia coli: location of the fumB gene and discovery of a new gene (fumC). J.Gen.Microbiol. 131:2971-2984
Printable Strain Report