Strain - BW25141/pKD20

Strain - BW25141/pKD20Strain BW25141/pKD20

Strain BW25141/pKD20

CGSC#: 7638
Strain Designation
Designation Source Preference
BW25141/pKD20 Wanner B.L. 1
Sex(Hfr,F+,F-,or F'): F-

11 Mutations:

Mutation	Location	Certainty	Display
DE(araD-araB)567	1.40	1	Δ(araD-araB)567
lacZ4787(del)(::rrnB-3)	7.81	1	ΔlacZ4787(::rrnB-3)
DE(phoB-phoR)580	8.96	1	Δ(phoB-phoR)580
LAM-	17.40	1	λ^-
galU95	27.82	1	galU95
uidA3(del)::pir+	36.48	1	ΔuidA3::pir⁺
recA1	60.80	1	recA1
endA9(del-ins)::FRT	66.57	1	endA9(del-ins)::FRT
rph-1	82.20	1	rph-1
DE(rhaD-rhaB)568	88.16	1	Δ(rhaD-rhaB)568
hsdR514	98.74	1	hsdR514

Comments:
- Send pKD46 instead. It is more efficient than pKD20. pKD20 sequence is GenBank AY048745.
Episome/Plasmid: pKD20
Plasmid Markers/Mutations:
araBp-gam-bet-exo
bla(ApR)
oriR101
repA101(ts)
araC
bla
References:
- Datsenko, KA, BL Wanner 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U.S.A. 97(12):6640-5.
Printable Strain Report